2 edition of Specificity of genetic transcription during embryogenesis of Xenopus laevis (Daudin) found in the catalog.
Specificity of genetic transcription during embryogenesis of Xenopus laevis (Daudin)
Written in English
|Contributions||Toronto, Ont. University.|
|The Physical Object|
|Pagination||vi, 77 leaves.|
|Number of Pages||77|
Reference genes are essential for gene expression analysis when using real-time quantitative PCR (RT-qPCR). Xenopus laevis is a popular amphibian model for studying vertebrate embryogenesis and. Xenopus tropicalis is now an anuran amphibian reference genome for vertebrate comparative genomics. It presents the same advantages as Xenopus laevis but has a smaller genome of Gbp and a shorter generation time .Moreover, while X. laevis is an allotetraploid derived from an allopolyploidization event, X. tropicalis is diploid [2, 3].Even though phylogenetic studies indicate that .
The Xenopus laevis TFIIIA promoter contains a motif that has been implicated in promoter activation in late-stage oocytes and contains the sequence () CACGTG (). A cDNA encoding a protein (B1) that binds to this element has been cloned from X. laevis and Xenopus borealis ovarian cDNA librari . We found that the LTR of λ-olt can function as the first exon and forms a transcript with a sequence that has a high homology with mars2 during Xenopus embryogenesis (Fig. 2a, .
Fig. 1. Characteristics of Xenopus laevis early development. (A) The different cell cycles and the external appearance of (a) the fertilized egg, and (b) two-cell, (c) mid-blastula and (d) early gastrula and Ab are views from the animal pole, Ac from the side and Ad from the vegetal pole. (Aa) Cycle 1 is approximately 90 minutes in length and has G1 and G2 phases. Gene expression during Xenopus embryogenesis measured at single-cell resolution. Science. Ap The dynamics of gene expression in vertebrate embryogenesis at single-cell resolution. Briggs JA, Weinreb C, Wagner DE, Megason S, Peshkin L, Kirschner MW, Klein AM. Click here to view article at Science. Click here to view on Pubmed.
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Ovsenek N, Williams GT, Morimoto RI, Heikkila JJ. cis-acting sequences and trans-acting factors required for constitutive expression of a microinjected HSP70 gene after the midblastula transition of Xenopus laevis by: Transcription of XLPOU3, a brain-specific gene, during Xenopus laevis early embryogenesis.
Baltzinger M(1), Relaix F, Remy P. Author information: (1)CNRS/UPRMécanismes Moléculaires de la Division Cellulaire et du Développement, Strasbourg, France. [email protected] by: LFB1 (HNF1) is a tissue-specific transcription factor found in the livers, stomachs, intestines, and kidneys of vertebrates.
By analyzing the promoter of the Xenopus LFB1 gene, we identified potential autoregulation by LFB1 and regulation by HNF4, a transcription factor Cited by: of HNF1alpha, a tissue-specific transcription factor with an expression pattern overlapping that of HNF4.
We have now identified a second Xenopus HNF4 gene, which is more distantly related to mammalian HNF4 than the previously isolated gene.
This new gene was named HNF4beta to distinguish it from the known HNF4Cited by: Shastry BS, Honda BM, Roeder RG () Altered levels of a 5S gene-specific transcription factor (TFIIIA) during oogenesis and embryonic development of Xenopus laevis.
J Biol Chem– J Biol Chem– We have carried out a large-scale, semi-automated whole-mount in situ hybridization screen of cDNA clones in Xenopus laevis embryos. We confirm that differential gene expression is prevalent during embryogenesis since 24% of the clones are expressed non-ubiquitously and 8% are organ or cell type specific marker genes.
Unfertilized eggs of many species contain large amounts of maternal mRNA that are used to support protein synthesis during the first few hours of development, before the onset of embryonic transcription.
We have examined the accumulation of nonpolysomal maternal RNAs in polysomes after fertilization in Xenopus laevis by measuring the distributions of specific sequences in nonpolysomal and. Xenopus oocyte 5S rRNA genes. An increase in histone H1 content specifically restricts TFIIIA-activated transcription, and a decrease in histone HI within chromatin facilitates the activation of the oocyte 5S rRNA genes by TFIIIA.
Studies with amphibian embryos have contributed major insights into the molecular basis of induction processes and the formation of germ layers during vertebrate embryogenesis. Primary signals that have been identified as growth factors or growth factor-related ligands act as inducing factors on their target cells and, by a change of the genetic program, evoke a specification of the cellular.
Kdm3a serves roles during various biological and pathophysiological processes, including sper-matogenesis and metabolism, determination of sex, androgen receptor‑mediated transcription and embryonic carcinoma cell differentiation.
In the present study, physiological functions of Kdm3a were evaluated during embryogenesis of Xenopus laevis. However, the expression of major tissue-specific markers did not change when analyzed by reverse transcription–polymerase chain reaction (RT–PCR).
In conclusion, diclofenac treatment can promote teratogenicity that results in morphological anomalies, but not disrupt the developmental tissue arrangement during Xenopus embryogenesis. DEVELOPMENTAL BIOLOGY() Regulation of the Xenopus laevis Transcription Factor IIIA Gene during Oogenesis and Early Embryogenesis: Negative Elements Repress the 0-TFIIIA Promoter in Embryonic Cells SAMUEL L.
PFAFF, ROBERT K. HALL, GUY C. HART, AND WILLIAM L. TAYLOR School of Medicine. Studying genes involved in organogenesis is often difficult because many of these genes are also essential for early development.
The allotetraploid frog, Xenopus laevis, is commonly used to study developmental processes, but because of the presence of two homeologs for many genes, it has been difficult to use as a genetic model.
Few studies have successfully used CRISPR in amphibians, and. Xenbase Gene ID: XB-GENE XB-GENE XB-GENE Gene Symbol: gata4: gata4.L: gata4.S: Chromosome: scaffold_13 chr5L chr5S. Wormington WM, Brown DD. The transcription of 5 S RNA genes during oogenesis results in the storage of sufficient 5 S RNA in ribosomes to support subsequent embryogenesis.
Xenopus oocytes of all stages synthesize oocyte-type 5 S RNA. A generalized repression of transcription occurs at meiosis and is maintained throughout early cleavage. Detection of the cyclin D1-specific mRNA during early embryogenesis in Xenopus laevis revealed that expression was induced soon after the midblastula stage but not during the cleavage stage, and.
Highlights We examine the role of Williams Syndrome Transcription Factor (WSTF) in development. WSTF plays a major role in neural and non-neural development in Xenopus. WSTF is critical for normal neural crest function during vertebrate development.
WSTF is necessary for normal expression of BMP4, SHH, PAX2, EPHA4 and SOX2. WSTF haploinsufficiency may explain many. In addition, during mammalian embryogenesis, Eomes is crucially required in extraembryonic tissues that are specific for intrauterine development.
This chapter mainly focuses on mammalian development of mouse; however, common functions shared among other vertebrate model system, such as embryos of zebrafish and Xenopus laevis, will be compared.
Xenopus laevis is a prime system for the study of embryogenesis in vertebrates. Both prelocalized information in the egg and inductive interactions between cells contribute to the ordered increase in complexity during development.
Embryonic induction, discovered in amphibians, is being studied intensely in Xenopus; recent work suggests a role for growth factors in this process. Somatic 5S RNA genes remain active following histone H1 addition. Changes in chromatin structure could have a dominant role in the regulation of class III gene expression during Xenopus embryogenesis.
M. Elhiti, C. Stasolla, in Brenner's Encyclopedia of Genetics (Second Edition), Abstract. Embryogenesis is a crucial phase of the plant life cycle, initiated with the fertilization of the zygote and culminated with the formation of a fully developed embryo.
The process is executed with defined patterns of cell divisions resulting in the proper formation of the body plan, which is.Xenbase Gene ID: XB-GENE XB-GENE XB-GENE Gene Symbol: gata6: gata6.L: gata6.S: Chromosome: Chr06 chr6L chr6S.Dworkin-Rastl E, Shrutkowski A, Dworkin MB. Multiple ubiquitin mRNAs during Xenopus laevis development contain tandem repeats of the 76 amino acid coding sequence.
Cell. Dec; 39 (2 Pt 1)– Pestell RQ. Microtubule protein synthesis during oogenesis and early embryogenesis in Xenopus laevis. Biochem J. Mar; (3)–